Imaging of Brain Tissue by Acoustic Microscopy

Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy

The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey ...

Ultrasonic tissue characterization of infarcted myocardium by scanning acoustic microscopy.

The purpose of this study was to ultrasonically characterize infarcted human myocardial tissue at the microscopic level by scanning acoustic microscopy. Infarcted myocardial specimens from ten cases with acute myocardial infarction were studied. Specimens were formalin fixed, paraffin embedded and sectioned to 10-micron thickness. A specially developed scanning acoustic microscope system, opera...

Local Study of Thin-Film Adhesion by Surface Acoustic Waves and Subsurface Acoustic Microscopy Imaging

Four thin-film adhesion characterization methods are proposed. Two of them are surface acoustic wave based and the two others emphasize the complementary nature between acoustic microscopy, scratch test and tensile experiments. A continuous wave scanning acoustic microscope was used to propagate surface modes in the specimen and to measure the surface acoustic wave velocity as a function of the...

Acoustic microscopy by atomic force microscopy

We have constructed an atomic force microscope enabling one to image the topography of a sample, and to monitor simultaneously ultrasonic surface vibrations in the MHz range. For detection of the distribution of the ultrasonic vibration amplitude, a part of the position-sensing light beam reflected from the cantilever is directed to an external knife-edge detector. Acoustic images taken on the ...

Brain Imaging with Multiphoton Microscopy